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 การประชุมวิชาการHigh temperature acetic acid fermentation with thermotolerant acetic acid bacteria and some other application with engineered acetic acid bacteriaผู้แต่ง: Toulaphone Keokene, Toshiharu Yakushi,  Dr.Gunjana Theeragool, Associate Professor , Watchara Kanchanarach, Uraiwan Tippayasak, Dr.wilawan Sintuprapa, Lecturer , Kannipa Tasanapak, Pattaraporn Rattanawaree, Shinsuke Fujiwara, Kazunobu Matsushita, การประชุมวิชาการ: 
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Molecular Taxonomy of Acetobacter syzygii SKU19 and Characterization of Its Acetic Acid Adapted Strains) ผู้เขียน:ดร.วิลาวัลย์ สินธุประภา, อาจารย์, ดร.กัญจนา ธีระกุล, รองศาสตราจารย์, ดร.วิเชียร ยงมานิตชัย, อาจารย์ , Pattana Srifah-Huehne, Kazunobu Matsushita สื่อสิ่งพิมพ์:pdf AbstractAcetobacter species are one of the most suitable bacteria for the proteomic analysis of proteins involved in stress response or adaptation. Among 129 isolates of thermotolerant acetic-acid bacteria, two isolates SKU19 and SKU123 were selected as an acetic-acid sensitive and acetic-acid resistant strain, respectively. Molecular taxonomy of these isolates indicated that SKU19 and SKU123 were Acetobacter syzygii and A. pasteurianus, respectively. To elucidate an adaptive response to acetic acid, acetic-acid-adapted strains were isolated from sequential cultivations of the acetic-acid sensitive strain, A. syzygii SKU19, in a medium containing 1% acetic acid. The adapted variants could be divided into two groups based on their growth and ability to further oxidize acetate. The first group consisted of cells with increased overoxidation (rapid acetate oxidizers), while the second group contained cells with increased stability to acetate (slow acetate oxidizers). The membrane-bound quinoprotein alcohol dehydrogenase (PQQ-ADH) and aldehyde dehydrogenase (ALDH) activities of these adapted strains were higher than those of the wild type. The result corresponded well with the increased amount of protein with a molecular mass of 72-80 (subunit I) and 44-54 kDa (subunit II) when the organism was cultivated in a medium containing acetic acid. This study confirmed that acetic-acid resistance in aceticacid bacteria is conferred by several mechanisms, such as acetate assimilation and PQQ-ADH functions. |
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ผลงานตีพิมพ์ในวารสารวิชาการThermal adaptation of acetic acid bacteria for practical high-temperature vinegar fermentationผู้แต่ง:Nami Matsumoto, Naoki Osumi, Minenosuke Matsutani, Theerisara Phathanathavorn, Naoya Kataoka, Dr.Gunjana Theeragool, Associate Professor, Toshiharu Yakushi, Yasushi Shiraishi, Kazunobu Matsushita, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการRelocation of dehydroquinate dehydratase to the periplasmic spaceimproves dehydroshikimate production with Gluconobacter oxydansstrain NBRC3244ผู้แต่ง:Kentaro Nakamura, Kakeru Nagaki, Minenosuke Matsutani, Osao Adachi, Naoya Kataoka, Yoshitaka Ano, Dr.Gunjana Theeragool, Associate Professor, Kazunobu Matsushita, Toshiharu Yakushi, วารสาร:  
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